In vitro Antibacterial Activity of Aquatic Garlic Extract, Apple Vinegar and Apple Vinegar - Garlic Extract combination

Dr. Nada KhazalKadhim Hindi


Background: Garlic  (Allium  sativum)  has  had  an  important  dietary  and  medicinal  role  for  centuries.  It  is  a  large annual plant of the Liliaceae family. Garlic is used in traditional medicine for infectious disease and some other cases.

Aims: The study aims at determining the antibacterial activity of of  aquatic garlic extract, apple vinegar and apple vinegar - garlic extract combination against some bacterial isolates.

Methods: The  antibacterial  effects  of  aqueous  garlic  extract, apple vinegar, and apple vinegar- garlic  extract combination   against  9  Gram-positive and 5 Gram-negative  bacterial isolates,  including Staphylococcus aureus, Staphylococcus epidermidis,  Streptococcus pyogenes,  Enterococcus feacalis,  Streptococcus pneumoniae, Pseudomonas aeruginosa, Pseudomonas fluresence,  Enterobacter aerugenes, Klebsiella pneumoniae, Escherchia coli,  Salmonella typhi,  Proteus mirabilis, Proteus vulgaris, and Acinetobacter,  all of them were  studied. 

Results: Antibacterial activity of  aqueous  garlic  extract  at 50%  concentrations  by  well-diffusion method was characterized by inhibition zones of 5 Gram-positive  and  9  Gram-negative  pathogenic bacteria.  The  maximum  zone  of  inhibition of  aqueous  garlic  extract   was observed  in Salmonella typhi and  the  minimum  was observed  for Proteus  sp. All organisms tested were highly sensitive to apple vinegar- garlic extract combination, whereas all organisms tested were slightly sensitive to apple vinegar.

Conclusions: In  summary,  the  aqueous  garlic  extract showed  a  wide  spectrum  activity  and  appears  to satisfy  all  of  the  criteria  for  antibacterial  agents.  These  results  suggest  that  garlic  can  be  used  to protect  food  and  reduce  the  risk  of contamination from pathogenic microorganisms.


Garlic; Apple Vinegar; Apple Vinegar - Garlic Extract combination; and antibacterial activity.

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